The next week, we ran two gel electrophoresis on our fish DNA, one for our gDNA and one for our PCR product. The agarose block was prepared for us already (well for the first one) and all we had to do was pipette our sample into the wells and run the gel electrophoresis for about 10 minutes at 145V. To prepare out samples, we pipetted 2 microlitres of loading dye on a piece of parafilm and pipetted 3 microlitres of our DNA onto it. We repeated this step for our PCR product BUT we reused our old agarose gel by melting it and letting it cool and set. After determining which PCR worked, we did an Exosap PCR Clean up. We first made a master mix per table consisting of water, buffer, Exo and SAP, and then we pipetted our PCR product into a PCR tube and pipetted 12.5 microlitres of our master mix in the tube. After this, we put the tubes in a thermocycler and ran the program and after it’s done, we put it in the freezer.

For my gel electrophoresis, only 2/3 of my fish samples worked. This is probably because I only realized till the end of the first lab that I was using the pipette wrong so I probably have the wrong volumes used.