Plant DNA PCR 3

We ran our PCR results of the ISSR from last week using a gel electrophoresis to determine which primers worked the best. Not surprisingly, ours didn’t work and the best two primers were Omar and 17898. We used our own original samples from the first time (mine was PSF01-05). And we basically did the same thing as last week: Create 2 mastermixes, one for Omar, and the other for 17898. We got our two strips of tubes of 5), pipetted 1 microlitre of our samples into each tube and add in 19 microlitre of the MasterMix to each respective tubes and ran a PCR.

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