From all the previous labs, we now have 41 lupine samples from 13 different geographic regions (photo below).

We created a consensus sequence using forward and reverse reads using a program called Geneious. To do so, we select both the forward and reserve reads and did a “De novo assemble” and edited the sequences and extracted the consensus sequences. We did this for all 41 lupine samples. We then ALL of the consensus sequences and used a MrBayes analysis to create a phylogenetic tree with a minimum of 1.5m generations, minimum burn in of 100,000, frequency of 500 and using Lcham as an outgroup and HKY85 as the substitution model.

My tree had a huge polytomy and two clades, with one support value of >0.85. Two of them were from the same georaphic range, and two were not.