On Tuesday, 09/17/2019 at 02:15 PM, we filled the gel electrophoresis lane table for each of our samples, I decided to place my samples with ID numbers EO 1, EO2, EO3 and EO4 to 5th, 6th 7th and 8th lanes respectively from left to right. Then, we started to undergo the procedure for the Electrophoresis of PCR products. I got my PCR tubes and thawed them.  I pipetted 3ul of my PCR products into its own dye dot on a sheet of parafilm. I loaded 5ul of my dots into the gel. We ran the gel at 130 volts for 30 minutes. After that, we moved to the clean-up procedure of PCR products for sequencing known as ExoSap. I carefully labeled new 0.2 ul PCR tubes with each of my sample codes. We determined the number of PCR clean-ups as 18. We calculated that we needed 191 ul of H20, 22.5 ul of 10x buffer, 7.9 ul of SAP and 4 ul of Exo. I put my reagents on ice and pipetted 7.5 ul of each of my PCR products into a clean, labeled 0.2 ul PCR tube. We made the ExoSap master mix and kept the reagents on ice while it is made. I pipetted 12.5 ul into each PCR product tube and placed my tubes in a thermocycler and then Prof. Paul started the EXOSAP program.