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ISSR Genomics

Lab Entry 13 – Genomics For this lab, analysis was done of the lupin plant DNA collected and utilized in previous posts. To run analysis, first a tutorial was completed in Geneious on reference-based assembly and de novo assembly. This… Continue Reading

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Plant PCR II

Lab Entry 10 – Plant PCR II Plant DNA PCR…Round 2 Due to some unfortunate results from the previous attempt at plant DNA PCR, the experiment was repeated. The primers being used were from the chloroplast gene psbA, were the… Continue Reading

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Geneious II

Lab Entry 7 – Geneious Part II Using the sequences acquired from the last lab entry (30 sequences in total), phylogenetic trees were created using various programs within Geneious. The first step to creating the trees was to align all… Continue Reading

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Geneious

Lab Entry 6 – Geneious Gene Sequencing and Analysis With the completion of DNA replication and running multiple PCRs, results of gene sequences were received in which analysis could take place. A program, Geneious, allows for this analysis by comparing… Continue Reading

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Field Trip II

Lab Entry 5 – Field Trip II   For the second filed trip the class took, we ventured to the Limantour Trailhead area in Point Reyes National Seashore. This location was approximately 1.5 hours north of San Francisco via driving. Lupinus arboreus… Continue Reading