LEXLY'S LAB NOTEBOOK

Molecular Ecology

DNA Extraction with modified Alexander et al. tube protocol

For the Plant DNA extraction lab a modified Alexander et al. tube protocol was followed. Filtered tips were used throughout the entire lab. Form the distributed samples, each person was assigned three different plant samples. Along with the samples 2.0… Continue Reading →

Phylogenetic Inference Lab- October 10th

On the day the lab pertained to phylogenetic inferences we arrived with an alignment of COI sequences including our own sushi DNA and 25 sequences form NCBI obtained through Geneious. The protocol instructed us to clean up all the sequences… Continue Reading →

Introduction to Geneious

On October 3rd we were introduced to the Geneious software program. The lab revolved around the interpretation of the sushi sequence we collected the durning the last laboratory session. Since we had already downloaded the program onto out laptops before… Continue Reading →

Molecular Ecology Field Trip to see the Purple-flowered Lupine and Mimulus Cardinals

On September 26, 2018 the class took a trip to Mt. Tamalpais to see the  Purple-flowered Lupine and Mimulus Cardinals. The first stop to led us to hike down a steep hill to a small stream. Unfortunately we did not… Continue Reading →

Lupinus Arboreus Plant Field Trip

On the 19th of September the class took a trip to the coast south of USF to see the Lupinus arboreus population. The first stop was on atop a hill side parallel to the ocean. There the class observed a… Continue Reading →

Lab #3: PCR Clean Up Protocol

First the PCR products from lab two were run through electrophoresis. The content in the five sample tubes were pipetted onto a row with five dots of Loading Dye. When each sample was pipetted the pipette tip was exchanged for… Continue Reading →

DNA Barcoding Project: Lab 2 Extracting and Amplifying Sushi DNA

Initially the different samples of fish were given a unique ID code that was recorded on the “Animal Tissue DNA Extraction” data sheet along with the name of species given by the restaurant. Five sushi samples were labeled “LMC01” with… Continue Reading →

DNA Barcoding Project- The Collection of Sushi

Restaurant: Fujiyama-ya Type: Japanese Location: 1234 Noriega St, San Francisco, CA 94122 Sushi Name From Left to Right: Tuna, Yellowtail, Salmon, Escolar, and Mackerel With: Wasabi and Ginger The Receipt: We ended up purchasing udon along with the sushi! Evidence: A video… Continue Reading →

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