We conducted a double digest restriction associated DNA study on M. Guttatus. The first step was collecting samples which we did on two separate field trips that can be read about here: https://usfblogs.usfca.edu/rgutierrezvallejo1/ Next we extracted DNA from samples we… Continue Reading
In today’s Lab 2 distinct PCR reactions were performed. One, labeled Test PCR, was used to create a library for M. Gattatus samples. The second PCR was to actually generate data on the M. Gattatus samples. For the final PCR… Continue Reading
For today’s lab we did DD-RadSeq of our samples of M. Gattatus plus some samples taken by Dr. Paul’s research Lab. For Dr. Paul’s samples: Adapters were thawed out on ice. Add 1µL of working stock of EcoRI adapter straight… Continue Reading
In this lab a master mix was created to help the PCR reaction. The following was added to the master mix: 146.9 µL ddH2O 22 µL 10x buffer 22 µL MgCl2 11 µL BSA 2.2 µL dNTPs 2.2 µL of… Continue Reading
In this lab we ran an electrophoresis of PCR for M. Gattatus Samples from Lab 8. Thaw out PCR tubes from previous labs. Using a sheet of parafilm, dot out 12 dots, or the number necessary for each PCR sample… Continue Reading
In this Lab we prepared sampled of M. Gattatus for Gel electrophoresis by doing the following: Collect 3 2.0 mL tubes and label each with respective sample code and add 3 2 mm stainless steel beads to each tube. Take… Continue Reading
In this week’s lab, we continued using Genious, but this time in an attempt to create a series of phylogenetic trees using our alignments of 25+ species. In the particular alignment we made we used a ray or shark species… Continue Reading
During this week’s lab we were introduced to a variety of way we can manipulate a gene sequence using a program called Geneious. With this program we can edit and change individual nucleotides on our sequence, as well as compare… Continue Reading
Field Trip Log: Upon arriving at side of hill, we viewed the fresh spring water that ran down the hill. This stream of clean water ran out the side of the mountain and created a miniature creek upon which many… Continue Reading
Part 1: Electrophoresis of PCR Product Thaw out PCR tubes from previous labs. Using a sheet of parafilm, dot out 16 dots, or the number necessary for each PCR sample plus your negative control, each dot should be about 1µL.… Continue Reading
