PCR Reactions

On Tuesday 11/05/19 at 2:30 PM, we started our lab session which involved PCR reactions. We prepared a master mix using 214uL of ddH2O, 32uL of 10x buffer, 32uL of MgCl2, 16uL of BSA, 3.2uL of dNTPs, 3.2uL of F-primer, 3.2uL of R-primer and 1uL of Taq. Then, I labeled 4 tubes with the following tags; D4, D5 and D6 and added 19 ul of master mix along with 1uL template from EO cATB and JP MW 03 and SHOR 004 to each tube respectively. Finally, I vortexed the tubes and stored them in ice.

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