Lab 9: Gel Electrophoresis

October 29, 2019

We began our electrophoresis lab by gathering our Mimulus guttatus DNA samples from our last lab. I first started by dotting out 16 loading dye dots on a sheet of parafilm. I loaded 1 µl dye dots using a 20 µl micropipette. We then pipetted 3 µl of each PCR product into its own dot. After all the product was pipetted into the loading dyes, we then set the pipette to 5 µl and loaded the dots into each well. We then ran the gel at 130 volts for 30 minutes.



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