We constructed a double digest restriction associated DNA study on Mimulus guttatus. The first step was collecting samples, which we did on two field trips which can be read about here and here . Next, we extracted DNA from the samples… Continue Reading
After double digesting and ligating an adapter to the DNA, we did a test PCR. We did this to test for successful library construction of the samples. First, we made a master mix for the RADSeq using the following: Master… Continue Reading
Our next step for this lab was to do DD-RADSeq. First, we double digested our DNA samples. To do this, we followed the following steps: Double digested 100-1000 ng of high quality genomic DNA with selected restriction enzymes, using a… Continue Reading
