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Lab entry 12

After double digesting and ligating an adapter to the DNA, we did a test PCR. We did this to test for successful library construction of the samples. First, we made a master mix for the RADSeq using the following: Master… Continue Reading

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Lab entry 11

Our next step for this lab was to do DD-RADSeq. First, we double digested our DNA samples. To do this, we followed the following steps: Double digested 100-1000 ng of high quality genomic DNA with selected restriction enzymes, using a… Continue Reading

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Lab 9 entry

Once my DNA was extracted, I prepped it for gel electrophoresis. First, we dotted ~2mL of blue tracking dye on a piece of parafilm. Then, I put ~3mL of each extracted DNA on top of a dot, changing tips every… Continue Reading

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Lab 6 Entry

Using Geneious, we were able to match our fish DNA to DNA in the database. To do this we first copied the reverse sequence from the ‘Fish barcode Reverse Reads’ folder and pasted it into the ‘Forward Reads’ folder. Then,… Continue Reading