Allyson Luber

November 5, 2019

• In this lab session we spent the majority of our time preparing the master mix to put into our small PCR tubes
• The master mix ratio had to be 19.00  μL for each reaction, but since we are a table of 3, we multiplied each ingredient to 11 to produce enough Master mix for each person

Tubes/Specimen ID

1.  DIRA-012
2. CHIM-007
3. CATB-RG
4. RWCKAJC
5. MAPLAJC
6. CATB-EVR
7. AEL-CATB
8. MAPL-001
9. RDRK-003

(Tubes 1-3 is Ricky’s; 4-6 is Eli’s; 7-9 is mine)

Master Mix: PCR Reactions (20  μL)

Ingredients / per rxn ( μL) / rxn x 11 ( μL)

• ddH2O / 13.36 / 146.9
• 10x buffer / 2.00 / 22
• MgCl2 / 2.00 / 22
• BSA / 1.00 / 11
• dNTPs / 0.20 / 2.2
• F-Primer (Miri 70) / 0.20 / 2.2
• R-Primer (Miri 70) / 0.20 / 2.2
• Taq / 0.04 / 0.41
• Template / 1.00 / n/a

Total: 19.00 per rxn / 11 rxn’s (our group’s total): 208.91  μL

• With the master mix finished, we pipeted 19.00  μL to each of our individual rxn’s