This week, we worked with the program Geneious to sequence our fish DNA. Unfortunately, the fish samples that I PCR’d did not produce a useable barcode sequence. For this lab, I used barcodes from a previous student. The samples were EZ02 and EZ03. When she collected the sushi, the student was told that EZ02 was red snapper and EZ03 was yellowtail. I used Geneious to assemble, edit and BLAST these DNA sequences, and found the results of the species barcodes. EZ02 was not red snapper as advertised, it was Oreochromis niloticus, or Nile tilapia. The other sample, EZ03 was Seriola quinqueradiata, or yellowtail, so this one was correctly labeled.
The EZ02 alignment I built had only one polymorphism, in column 663. The EZ03 alignment had seven polymorphisms, found in columns 5, 6, 7, 11, 12, 15, and 201.