November 4th, 2019 – Lab 10

This lab, we added microsatellites to the previously prepared samples of M. guttatus in order to process the DNA and to understand genetic diversity within M. guttatus.

Materials

  • ddH2O
  • 10x buffer
  • MgCl2
  • BSA
  • dNTPs
  • F-Primer (MIRI70)
  • R-Primer (MIRI70)
  • Taq
  • Template
  • Ice

11 PCR reactions were prepared to accommodate 9 samples of M. gutattus. All components of the PCR reaction were kept on ice. 146.9 uL of ddH2O, 22 ul of 10x bufffer, 22 ul of MgCl2, 11 uL of BSA, 2.2 uL of dNTPs, F-primer, and R-primer, and 0.44 uL of Taq were added to make approximately 19 uL per reaction. 1 uL of each sample was added to an individual tube and vortexed with the reaction mix to be processed.

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