DNA Barcoding Analysis I

To analyze the DNA that we got, we used an app called Geneious. Seeing how only one of my DNAs worked, I was presented with two other DNA samples. We opened our DNA reads on Geneious and combined the reverse and forward sequences using a function called “De Novo assembly” to assemble a sequence. We edited the sequence by erasing any discrepancies or ambiguity. We used this sequence to generate a consensus sequence and BLAST-ed the sequence, which gives us the scientific name of the species. A google search of the scientific name would tell us the simpler name of the species. After this, we built an alignment by taking some hits from the BLAST results and the consensus sequence and did a “multiple align”.

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