We received 5 new samples – I got GWC04, PRD04, PRM04, PSR04 and PHO04 and used two molecular markers – ISSR and psbA (since ours failed last week). For the ISSR, we tested out one of four primers (HB10, 17898, Omar, and 844).

The methods for preparing the template DNA is the same (https://usfblogs.usfca.edu/changmargarette/2017/10/23/plant-dna-pcr/)

To create the MasterMix of the ISSR, pipette into a centrifuge tube:

• 12.5 microlitres distilled water
• 3 microlitres buffer
• 1 microlitres BSA
• 2 microlitres dNTP
• 0.25 microlitres primer – 844.
• 0.25 microlitres Taq.

To create the MasterMix of psbA: (https://usfblogs.usfca.edu/changmargarette/2017/10/23/plant-dna-pcr/)

Pipette the 19 microlitres of the mastermix into each respective tube.