Protocol:
Used extracted DNA from the first Plant DNA extraction lab
GWC01-GWC05
- Loaded agarose gels with 3 microliters of template and 1 microliter of loading dye on parafilm
- ran for about 25 minutes at 130 v in the first bottom row of gel 2
Preparing template DNA:
- used two .2 ml pcr tubes
- pipetted 1 microliter of each sample into respective tube
- created master mix of (per tube) using primers of pSBA and ITS genes
- ddh20 – 15 microliters
- 10x buffer+mg – 2 μL
- BSA – 1 μL
- dNTPs – .20 μL
- f primer – .20 μL
- r primer – .20 μL
- Taq – .04 μL
- template – 1 μL
- Pipetted master mix into respective tubes and ran on thermocycler