Protocol

1. Added 1 microliter of loading dye to PCR products
2. Ran PCR products in 1.3x agarose loading gel
3. Ran for about 1 and a half hours at 60v
4. Imaged gels to determine which ISSR’s work best
   1. Decided to use 17898 and OMAR
5. Made 1:10 dilution of Plant DNA (10 microliters DNA, 90 Microliters water) from GWC samples
6. Made two master mixes with
   1. ddh20        12.5 microliters
   2. 10x buffer + MH     3 microliters
   3. BSA            1 microliter
   4. dNTPs        2 microliters
   5. Primers     .25 microliters (either OMAR or 17898)
   6. tax              .25 microliters
   7. template    1 microliter
7. Ran on thermocycler