To begin the protocol we made two sets of 1:10 dilutions of our plant samples form the previous week. Each Lupinus arboreus sample was placed in a labeled 1.5 mL tube with its unique label. 45 μL of water and 5 μL of plant sample were placed in each tube. The dilutions were collected and distributed to the class.

Next, the procedure instructed us to use interspersed-simple-sequence-repeat primers to group the plant samples. Each individual received 15 samples and each table created their own master mix. First, 2 0.2ml 8-tube strips were obtained and the sample IDs of each sample were recorded on a sample ID sheet. Each tube was labeled with it own ID and numbered 1-16. The primer name, OMAR, was written on each tube as well. Using different filter tips for each sample, 1μL of each sample DNA was pipetted into its designated tube. The tubes were sealed.

While we conducted this step, one of the members of the table created the master mix for 60 samples. The calculations were done to use the right amount of components for 60 samples. The tube in which it was created was labeled master mix. Master mix was added to each sample tube and sealed tightly.

Image: Data Sheet and Master Mix Calculations