This week we ran the PCR reactions for our Mimulus guttatus project – materials shown below. We made enough Mastermix for 18 reactions even though we only had 12- just to make sure we had enough.
|Ingredients||Per Reaction||[18 reactions]|
|Taq||0.04||.72 àRounded to .80 (for pipette)|
We labeled our tubes as shown below:
|QS 1 MM||MM1|
|QS 2 MM||MM2|
|QS 3 MM||MM3|
|QS 4 OY||OY01|
|QS 5 OY||OY02|
|QS 6 OY||OY03|
We added 19 microliter of Mastermix to each of the 12 reactions, mixed them on the vortex then Prof. Paul ran the PCR reaction for us.