Protocol:

Used extracted DNA from the first Plant DNA extraction lab

GWC01-GWC05

1. Loaded agarose gels with 3 microliters of template and 1 microliter of loading dye on parafilm
2. ran for about 25 minutes at 130 v in the first bottom row of gel 2

Preparing template DNA:

1. used two .2 ml pcr tubes
2. pipetted 1 microliter of each sample into respective tube
3. created master mix of (per tube) using primers of pSBA and ITS genes
   1. ddh20 – 15 microliters
   2. 10x buffer+mg – 2 μL
   3. BSA – 1 μL
   4. dNTPs – .20 μL
   5. f primer – .20 μL
   6. r primer – .20 μL
   7. Taq – .04 μL
   8. template – 1 μL
4. Pipetted master mix into respective tubes and ran on thermocycler