Allyson Luber
November 5, 2019
- In this lab session we spent the majority of our time preparing the master mix to put into our small PCR tubes
- The master mix ratio had to be 19.00 μL for each reaction, but since we are a table of 3, we multiplied each ingredient to 11 to produce enough Master mix for each person
Tubes/Specimen ID
- DIRA-012
- CHIM-007
- CATB-RG
- RWCKAJC
- MAPLAJC
- CATB-EVR
- AEL-CATB
- MAPL-001
- RDRK-003
(Tubes 1-3 is Ricky’s; 4-6 is Eli’s; 7-9 is mine)
Master Mix: PCR Reactions (20 μL)
Ingredients / per rxn ( μL) / rxn x 11 ( μL)
- ddH2O / 13.36 / 146.9
- 10x buffer / 2.00 / 22
- MgCl2 / 2.00 / 22
- BSA / 1.00 / 11
- dNTPs / 0.20 / 2.2
- F-Primer (Miri 70) / 0.20 / 2.2
- R-Primer (Miri 70) / 0.20 / 2.2
- Taq / 0.04 / 0.41
- Template / 1.00 / n/a
Total: 19.00 per rxn / 11 rxn’s (our group’s total): 208.91 μL
- With the master mix finished, we pipeted 19.00 μL to each of our individual rxn’s