Lab 12:
In today’s Lab 2 distinct PCR reactions were performed. One, labeled Test PCR, was used to create a library for M. Gattatus samples. The second PCR was to actually generate data on the M. Gattatus samples. For the final PCR… Continue Reading
Month: November 2019
Lab 11
For today’s lab we did DD-RadSeq of our samples of M. Gattatus plus some samples taken by Dr. Paul’s research Lab. For Dr. Paul’s samples: Adapters were thawed out on ice. Add 1µL of working stock of EcoRI adapter straight… Continue Reading
Lab 10
In this lab a master mix was created to help the PCR reaction. The following was added to the master mix: 146.9 µL ddH2O 22 µL 10x buffer 22 µL MgCl2 11 µL BSA 2.2 µL dNTPs 2.2 µL of… Continue Reading
Lab 9
In this lab we ran an electrophoresis of PCR for M. Gattatus Samples from Lab 8. Thaw out PCR tubes from previous labs. Using a sheet of parafilm, dot out 12 dots, or the number necessary for each PCR sample… Continue Reading
