First, I labeled 3 2.0 mL tubes with my sample codes (KRS1-3 respectively). I added 3 sterile 3.2-mm stainless steel beads to each tube. Then, I added a small amount of leaf tissue into each tube, cleaning the tweezers between… Continue Reading
I created an alignment of 25 COI sequences from Actinopterygii and my fish DNA barcode sequences, including one Chondrichthyes sequence as an out-group. I did this by searching COI and Actinopterygii/Chondrichthyes in the NCBI nucleotide program in Geneious. I chose… Continue Reading
Using Geneious, we were able to match our fish DNA to DNA in the database. To do this we first copied the reverse sequence from the ‘Fish barcode Reverse Reads’ folder and pasted it into the ‘Forward Reads’ folder. Then,… Continue Reading
On Tuesday, September 24, 2019, we traveled back to Mount Tamalpais, this time stopping near Muir Beach to collect and view samples of mimulus guttatus. We stopped at a natural spring to drink the water and observe the flowering mimulus… Continue Reading
